A Novel Technique for Fabricating Plastic Lab-on-a-Chip Devices with an Immobilized Enzyme
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Other Titles:Novel Dense CO2 Technique for Beta-Galactosidase Immobilization
Publisher:American Chemical Society - Biomacromolecules
Citation:Biomacromolecules 2008, 9, 1027–1034
Series/Report no.:2008 Edward F. Hayes Graduate Research Forum. 22nd
In this study we design new fabrication techniques and demonstrate the potential of using dense CO2 (i.e. high pressure carbon dioxide that possess a liquid-like density) for facilitating crucial steps in the fabrication of plastic lab-on-a-chip (LOC) micro-devices by embedding bio-molecules at temperatures well below the plastic’s glass transition temperature (Tg). The polymer polystyrene (PS) is the plastic that was used in this study and has a Tg of ~105oC. The Tg is the temperature at which the plastic becomes rubbery and deformable, below this temperature it is glassy and acts as a brittle solid. These new techniques are environmentally friendly and done without the use of a clean room. Carbon dioxide at 40oC and between 4.48 and 6.89 MPa was used to immobilize the biologically active molecule, beta-galactosidase (beta-gal), on the surface of PS micro-channels. To our knowledge, this is the first time dense CO2 has been used to directly immobilize an enzyme in a micro-channel. Beta-gal activity was maintained, and shown via a fluorescent reaction product, after enzyme immobilization and micro-channel capping by the designed fabrication steps at 40oC and pressures up to 6.89 MPa.
Engineering: 3rd Place (The Ohio State University Edward F. Hayes Graduate Research Forum)