Generation of Immunoprotection Against Squamous Cell Carcinomas by In Vitro Cultivation and a Possible Mechanism of Action
Creators:Jamasbi, Roudabeh J.
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Citation:The Ohio Journal of Science. v94, n1 (March, 1994), 14-23
The immunogenicity of individual diethylnitrosamine (DEN)-induced forestomach carcinomas in female BALB/c mice was investigated following in vitro and in vivo cultivation. Of the five transplantable tumor lines studied, (DEN1? DEN3, DEN6, DEN8, and DEN^ only two (DEN6 and DEN8) showed some degree of immunogenicity. DENX, E»EN3, and DEN9 were highly tumorigenic with very little immunogenic potency as judged by tumor transplantation-excision assay, Winn neutralization, and antibody binding tests. These three tumors grew rapidly and showed a high degree of malignancy. DENX and DEN3 also metastasized readily. Cell lines from DEN6 and DEN9 lost their tumorigenicity at the 5th and 50th passage of culture, respectively. Although DENa and DEN3 did not lose their tumorigenicity, the number of tumor cells required to produce tumors increased substantially and their ability to metastasize was lost. Tumor transplantation studies, with these cultured cell lines in normal and x-irradiated recipients, suggested that the decrease in tumorigenicity may be immunologically mediated. Mice immunized with the in vitro lines demonstrated transplantation resistance against the respective in vitro and in vivo lines. The treatment of in vivo or in vitro propagated cells with periodic acid or neuraminidase enhanced antigen-antibody binding significantly. The effect of these chemicals became less pronounced as in vitro culture continued. It appears that during in vivo cultivation the antigenic determinants are masked or modulated by some glycoprotein or glycolipid molecules which render them non-, or very weakly, immunogenic.
Author Institution: Departments of Medical Technology and Biological Sciences, Bowling Green State University
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