Effect of Sodium Bisulfite on Peroxidase Activity and Electrolyte Leakage in Maize in Relation to Sporulation of Bipolaris maydis Race T
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Citation:The Ohio Journal of Science. v90, n3 (June, 1990), 71-76
In this study, we sought to determine whether the increases in peroxidase activity and electrolyte leakage induced in maize (Zea mays L.) leaves by sodium bisulfite were causally related to the sodium bisulfite-induced increases in sporulation of the pathogen Bipolaris maydis race T on infected maize leaves. Pretreatment of detached leaves of maize inbred W64 A with sodium bisulfite (500 |ig/ml) for 24 h in the dark at 28°C increased peroxidase activity in the Tms cytoplasm (susceptible) isoline compared with the N cytoplasm (resistant) isoline. No such differences in peroxidase activity between the two isolines were observed when detached leaves were pretreated with double distilled water. The sodium bisulfite-induced increase in peroxidase activity persisted even when leaves pretreated with sodium bisulfite were inoculated with R maydis race T and subsequently incubated for 48 h in the dark at 28° C. Similarly, pretreatment with sodium bisulfite caused a greater increase in electrolyte leakage as well as in sporulation on the leaves of the susceptible than on those of the resistant isoline when compared with leaves not treated with sodium bisulfite. Sodium bisulfite showed no effect on sporulation in vitro. Leachates from the susceptible isoline pretreated with sodium bisulfite also caused greater increase in sporulation than those from the resistant isoline pretreated with sodium bisulfite.
Author Institution: Department of Plant Pathology, Ohio Agricultural Research and Development Center and The Ohio State University
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