A Method for the Cultivation of Dispersed Epithelial Cells from Mouse Palatal Mucosa

Abstract

A cell dispersion method is described for establishing cultures of mouse palatal epithelial cells which are viable in primary cultures and subcultures for extended periods of time. The cells in vitro mainly displayed the normal morphologic characteristics of palatal epithelial cells. As demonstrated by labelling the cells with tritiated thymidine, the monolayers of epithelial cells possess a high rate of DNA synthesis. Karyotypes of the cultured cells show a high percentage of normal displays of chromosomal numbers typical of the mouse strain employed. The number of spontaneous aberrations observed is in agreement with previous investigations using other methods.