Effects of Illumination and Temperature on Metabolic Patterns of the C-Incorporation by the Moss, Dicranum Scoparium
Creators:Wu, Pei-Hsing Lin
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Citation:The Ohio Journal of Science. v76, n3 (May, 1976), 103-109
The moss, Dicranum scoparium, conditioned and maintained at temperatures ranging from 0° to 10°C, was subjected to acetate-2-14C incubation at 5° and 22°C in the presence or absence of added illumination. Patterns of 14C-incorporation into various non-protein fractions (including lipid-fatty acids) respired CO2, and alcoholic solubles were examined. The 14C-labeled metabolic products in amino acid-, organic acid-, and sugar-pools were separated and identified with column and thinlayer chromatographic techniques. Radioactivity increased in the sugar-pool under illumination regardless of incubation temperatures. The radioactivity in glucose remained unchanged regardless of illumination, but the formation of sucrose appeared to be light regulated. The combined regime of added illumination and raised temperature brought about an increase in activity for organic acid and a decrease in amino acid-pools although a sharp rise of 14C-glutamine was occurring in the latter pool. High light gave rise to more 14C-amino acids than those with a low light, regardless of incubation temperatures. Total level of activity in lipid-fatty acid pool remained unchanged during changes of illumination and incubation temperature, while its components, Lx and L2, fluctuated.
Author Institution: Department of Botany and Institute of Polar Studies, The Ohio State University
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