A Rapid Technique for the Detection of Nicotine in Developing Tobacco Seedlings
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Citation:The Ohio Journal of Science. v73, n6 (November, 1973), 353-356
A rapid and sensitive method for detecting alkaloids, in particular nicotine, from Nicotiana rustica tobacco seedlings up to 2 mm in length has been developed. Growing tissue is applied (squashed) directly onto silica gel plates for thin-layer chromotographic analysis. The sensitivity of this method permits the detection of quantities of nicotine as small as 0.4 microgram.
Author Institution: Department of Botany and Institute of Polar Studies and Department of Microbiology, The Ohio State University
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