Positional and Sequence Dependency of START Domains of the HD-ZIPIII Transcription Factor PHABULOSA in A. thaliana
MetadataShow full item record
Publisher:The Ohio State University
Series/Report no.:The Ohio State University. Department of Molecular Genetics Undergraduate Research Theses
To determine if the activity of the transcription factor PHABULOSA (PHB) is affected by the relative position and sequence of its two adjacent STAR-related lipid Transfer (START) domains, domain-swapped transgenes were heterologously expressed in Arabidopsis thaliana for biochemical analyses and phenotypic screening. qRT-PCR data from known PHB constructs containing domain swaps, as well as preliminary data from deletion mutants, suggest differential contributions from START1 and START2. Phenotype analysis of native plant lines shows that the chimeric PHB protein is nonfunctional and does not create the gain of function severe phenotype. The transcriptional regulation by the chimeric proteins assayed here suggests that both domains are needed for activity, as well as their relative position.
Academic Major: Molecular Genetics
Items in Knowledge Bank are protected by copyright, with all rights reserved, unless otherwise indicated.