The Ohio State University. School of Biomedical Science Honors Theses
mRNA research has heightened in prevalence due to the emergence of mRNA-based vaccines for the SARS-CoV-2 virus, but there is still a need for carriers to deliver future mRNA-based vaccines and therapies. The aim of this study was to develop and optimize formulations for novel lipid nanoparticles (LNP) to deliver mRNA in different cell lines. One novel LNP, CW51, was selected for formulation optimization via an orthogonal array. These optimized formulations were able to transfect JAWS II dendritic and C2C12 skeletal muscle cells with luciferase mRNA. These findings indicate that the lead LNP warrants further investigation into its potential in mRNA-carrying nanoparticles.