PHOTOCLEAVABLE QUANTUM DOT-GOLD NANOPARTICLE SYSTEMS FOR SUPER-RESOLUTION IMAGING

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dc.contributor.advisor Winter, Jessica
dc.creator Kevlich, Nikita
dc.date.accessioned 2013-05-01T16:18:00Z
dc.date.available 2013-05-01T16:18:00Z
dc.date.issued 2013-05
dc.identifier.uri http://hdl.handle.net/1811/54717
dc.description.abstract The ability to directly see structures and observe biological processes below the 200 nm resolution limit of traditional optical imaging methods would enable substantial advances in biology. Super-resolution imaging techniques use the bright/dark states of fluorescent probes and computer algorithms to image beyond that barrier by imaging probes in small groups rather than all at once. Quantum dots (QDs) have advantages over the florescent dyes and proteins currently used because of their increased brightness, stability, and resistance to photobleaching. However, they cannot be turned on and off stochastically. Here, we describe a QD-gold nanoparticle (AuNP) system that uses Förster (fluorescence) nonradiative energy transfer (FRET) for potential application in super-resolution imaging. When the composite is formed using a linker, it is dark, but when the linker is cleaved by light energy, QD fluorescence is restored. The initial conjugation was not successful because of instability of the AuNP. The AuNP was stabilized by using triethylene glycol mono-11-mercaptoundecyl ether, but no FRET was detected. Analysis with a transmission electron microscope demonstrated very few conjugated samples. FRET was tested using high concentrations of AuNPs and QDs, but without clear quenching. Future work would include using a shorter, reversible linker, such as molecules that change conformation upon UV light or shrinkable/stretchable polymers. en_US
dc.description.sponsorship National Science Foundation en_US
dc.description.sponsorship College of Engineering en_US
dc.language.iso en_US en_US
dc.publisher The Ohio State University en_US
dc.relation.ispartofseries The Ohio State University. Department of Chemical and Biomolecular Engineering Undergraduate Research Theses; 2013 en_US
dc.subject Nanoengineering en_US
dc.subject Super-resolution imaging en_US
dc.subject Quantum Dots en_US
dc.subject Gold Nanoparticles en_US
dc.subject FRET en_US
dc.title PHOTOCLEAVABLE QUANTUM DOT-GOLD NANOPARTICLE SYSTEMS FOR SUPER-RESOLUTION IMAGING en_US
dc.type Thesis en_US
dc.description.embargo No embargo en_US
dc.description.academicmajor Academic Major: Chemical Engineering en_US