Dissolved Organic Matter Contains Previously Unidentified Protein-like Fluorophores in Old Woman Creek

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Title: Dissolved Organic Matter Contains Previously Unidentified Protein-like Fluorophores in Old Woman Creek
Creators: Perez, Victor, Jr.
Advisor: Chin, Yu-Ping
Issue Date: 2012
Abstract: Dissolved organic matter (DOM) constitutes a significant carbon pool in the global carbon cycle and is influential in many other processes e.g., altering the fate of contaminants, acting as redox and pH buffers, etc. The ubiquitous dispersal of DOM in the aqueous environment originates from sources ranging from autochthonous (microbial) to allochthonous (terrestrial) precursors. The source of the DOM dictates its composition, which in turn impacts its reactivity in the environment. The isolation of DOM from natural waters is a common practice to preserve and concentrate DOM, yet the extraction method may significantly alter its composition. This study explores the characteristics of DOM collected from Old Woman Creek (OWC) National Estuarine Research Reserve, located in Huron, Ohio, isolated using PPL solid phase extraction (SPE). Previous DOM extraction methods such as C-18 or XAD-8 are limited to hydrophobic compounds, while the novel PPL SPE cartridges capture more polar and non-polar components. PPL and previously collected XAD-8 isolated OWC DOM was characterized using fluorescence spectroscopy. Fluorescence analysis revealed protein-like components not present in previously collected XAD-8 isolated OWC DOM, demonstrating the significance of extraction method on the composition and potential reactivity of isolated DOM.
Series/Report no.: The Ohio State University School of Earth Sciences. Shell Undergraduate Research Experience (SURE) Internships. 2012
Keywords: Dissolved Organic Matter
PPL Extraction
Sponsors: Shell Oil Corporation
Embargo: No embargo
URI: http://hdl.handle.net/1811/52989
Publisher: The Ohio State University
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