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A Novel Procedure for Qualifying and Quantifying Mono-, Di, and Trisaccharides by Derivatization and Gas Chromatography

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Title: A Novel Procedure for Qualifying and Quantifying Mono-, Di, and Trisaccharides by Derivatization and Gas Chromatography
Creators: Liu, Xiaxi
Advisor: Callam, Christopher
Issue Date: 2011-06
Abstract: The identification and quantification of carbohydrate content in aqueous solutions and biological systems are of great interest and importance. The polar nature and large sizes of sugars can cause difficulties in identification and separation processes. Problems include long retention times and degradation during separation procedures, such as column chromatography or gas chromatography (GC). Previous work in GC analysis of carbohydrates has shown quantitative results with high levels of noise due to decomposition and relatively poor detection limits. This project has developed methodology for an optimal quantification and identification procedure. We have derivatized carbohydrates to improve detection limits and resolution in standard GC and gas chromatography and mass spectrometry (GC/MS) analysis. This method has allowed for the identification and the separation of a synthetic mixture of 18 compounds, the qualitative identification and quantification of trace carbohydrate content in rat urine, and the analysis of cellular membrane permeability of a disaccharide. The necessary steps of this procedure involve converting carbonyl groups (aldehyde or ketone) into oximes, protection of the alcohols as trimethylsilyl ethers, concentration, and non-polar extraction. This study has the potential to make identification and quantification in biochemistry and medicinal chemistry less time-consuming and more accurate.
Embargo: No embargo
Series/Report no.: The Ohio State University. College of Biological Sciences Honors Theses; 2011
Keywords: gas chromatography
GC
mass spectroscopy
MS
carbohydrate
hyaluronic acid
urine
sugar
hydroxylamine
oxime
silylation
trimethylsilyl ether
trimethylsilyl imidazole
hexane
extraction
Sponsors: The Ohio State University Department of Chemistry
URI: http://hdl.handle.net/1811/48962
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Attribution-NonCommercial-NoDerivs 3.0 Unported