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Development of Inducible Constructs and Stable Cell Lines for Cbl Expression

Please use this identifier to cite or link to this item: http://hdl.handle.net/1811/45587

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Title: Development of Inducible Constructs and Stable Cell Lines for Cbl Expression
Creators: Abay, Solomon
Advisor: Lill, Nancy
Issue Date: 2010-06
Abstract: The E3 ubiquitin ligase Cbl plays a vital role in the trafficking of ligand-bound EGF-R through the endocytic pathway to its subsequent degradation. The RING finger tail region of the Cbl protein is thought to be critical to this process. Previous studies demonstrated that amino acid substitutions at position 431 or 434 of Cbl can disrupt the normal trafficking of EGF-R. Despite these previous findings regarding trafficking, the role of these mutations on EGF-R signaling has yet to be examined. In this study, we aim to begin to test the effects on signaling of the V431A and F434A mutations in a cellular context by developing pertinent plasmid constructs and generating stable, inducible cell lines for the signaling studies.
Embargo: No embargo
Series/Report no.: The Ohio State University. Department of Microbiology Honors Theses; 2010
Keywords: Cbl
EGF-R
Ring finger tail
inducible expression
pTRE
URI: http://hdl.handle.net/1811/45587
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Attribution 3.0 Unported