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RAPID QUANTIFICATION OF DIPICOLINIC ACID FROM SPORES TREATED BY PRESSURE-ASSISTED THERMAL PROCESSING USING INFRARED SPECTROSCOPY

Please use this identifier to cite or link to this item: http://hdl.handle.net/1811/38732

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dc.contributor.advisor Rodriguez-Saona, Luis E
dc.creator Prabhakar, Veena
dc.date.accessioned 2009-08-10T21:54:00Z
dc.date.available 2009-08-10T21:54:00Z
dc.date.issued 2009-04
dc.identifier.uri http://hdl.handle.net/1811/38732
dc.description Food, Agricultural, and Environmental Sciences (FAES): 1st Place (The Ohio State University Edward F. Hayes Graduate Research Forum) en_US
dc.description.abstract Endopores (or spores), which are produced by certain bacteria, are capable of surviving food processing and causing food spoilage and foodborne illness. Dipicolinic acid (DPA), which constitutes about 10% of the spores’ dry weight, plays a major role on wet heat resistance of spores. Current methods like chromatography and fluorometry that are used to quantify DPA are time-consuming, expensive and use hazardous solvents. Rapid methods to quantify the DPA levels in spores would enable simple and cost-effective approaches to study spore inactivation during thermal and non-thermal food processing. FTIR spectroscopic analysis was used to build a regression model that could be used for rapid prediction of DPA content released from treated Bacillus spores. Bacillus amyloliquefaciens FAD82 cultures were treated by pressure-assisted thermal processing with sampling at different time intervals. Aliquots (1mL) of the processed samples were centrifuged and the supernatant was analyzed using a fluorometer (reference method) to determine the released DPA concentration. Pellets were dried on a hydrophobic grid membrane filter and scanned using FTIR spectrometer. The collected spectra were correlated with the DPA concentrations obtained from the fluorometer to develop regression model based on partial least square regression analysis. The regression model was able to predict the DPA concentrations with a standard error of 6 µM. This method proved to be simple, rapid and accurate, without the need for expensive and hazardous chemicals. en_US
dc.language.iso en_US en_US
dc.relation.ispartofseries 2009 Edward F. Hayes Graduate Research Forum. 23rd en_US
dc.subject Bacterial spores en_US
dc.subject Dipicolinic acid en_US
dc.subject FTIR en_US
dc.subject Pressure-assisted thermal processing en_US
dc.title RAPID QUANTIFICATION OF DIPICOLINIC ACID FROM SPORES TREATED BY PRESSURE-ASSISTED THERMAL PROCESSING USING INFRARED SPECTROSCOPY en_US
dc.type Article en_US
dc.description.embargo A five-year embargo was granted for this item. en_US