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Mode of Action of Bacillus thuringiensis Cry1Ab Toxin: Role of Domain II Residue in Insertion into Insect Brush Border Membranes

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dc.contributor.advisor Dean, Donald H.
dc.creator Nair, Manoj S.
dc.date.accessioned 2008-05-16T00:47:02Z
dc.date.available 2008-05-16T00:47:02Z
dc.date.issued 2008-04
dc.identifier.uri http://hdl.handle.net/1811/32046
dc.description Biological Sciences: 2nd Place (The Ohio State University Edward F. Hayes Graduate Research Forum) en_US
dc.description.abstract Cry1Ab is a member of the family of crystal (Cry) toxins, produced by a soil bacterium Bacillus thuringiensis, and used globally as a commercial pesticide. A thorough knowledge of the mechanism by which the toxin acts is essential for preventing possible pest resistance to the toxin. While the upstream steps of the mechanism, including activation of the toxin in the alkaline midgut of the target lepidopteran insect followed by binding to its receptors are well understood, the final step of insertion of the toxin into insect brush border membranes remains controversial. The Umbrella and Penknife models hypothesize that the toxin partitions into the apical membrane of the insect midgut by insertion of only two alpha helices from domain I of the protein, alpha helices 4 and 5 in the case of the Umbrella Model and alpha helices 5 and 6 in the case of the Penknife Model. Neither model envisages membrane partitioning by domains II and III. In this study, we present data suggesting that mutations in domain II residue, F371 affect insertion of the whole toxin into Manduca sexta brush border membrane vesicles (BBMV). en_US
dc.language.iso en_US en_US
dc.publisher American Chemical Society en_US
dc.relation.ispartofseries 2008 Edward F. Hayes Graduate Research Forum. 22nd en_US
dc.subject Membranes, Toxin Insertion en_US
dc.subject Fluorescence, Blue-Shift en_US
dc.title Mode of Action of Bacillus thuringiensis Cry1Ab Toxin: Role of Domain II Residue in Insertion into Insect Brush Border Membranes en_US
dc.type Article en_US
dc.description.embargo A one-year embargo was granted for this item. en_US
dc.rights.cc Attribution-NoDerivs 3.0 Unported en_US
dc.rights.ccuri http://creativecommons.org/licenses/by-nd/3.0/ en_US
Attribution-NoDerivs 3.0 Unported This item is licensed under a Creative Commons License:
Attribution-NoDerivs 3.0 Unported