A Novel Technique for Fabricating Plastic Lab-on-a-Chip Devices with an Immobilized Enzyme

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Title: A Novel Technique for Fabricating Plastic Lab-on-a-Chip Devices with an Immobilized Enzyme
Creators: Ellis, Jeffrey
Advisor: Tomasko, David; Dehghani, Fariba
Issue Date: 2008-04
Abstract: In this study we design new fabrication techniques and demonstrate the potential of using dense CO2 (i.e. high pressure carbon dioxide that possess a liquid-like density) for facilitating crucial steps in the fabrication of plastic lab-on-a-chip (LOC) micro-devices by embedding bio-molecules at temperatures well below the plastic’s glass transition temperature (Tg). The polymer polystyrene (PS) is the plastic that was used in this study and has a Tg of ~105oC. The Tg is the temperature at which the plastic becomes rubbery and deformable, below this temperature it is glassy and acts as a brittle solid. These new techniques are environmentally friendly and done without the use of a clean room. Carbon dioxide at 40oC and between 4.48 and 6.89 MPa was used to immobilize the biologically active molecule, beta-galactosidase (beta-gal), on the surface of PS micro-channels. To our knowledge, this is the first time dense CO2 has been used to directly immobilize an enzyme in a micro-channel. Beta-gal activity was maintained, and shown via a fluorescent reaction product, after enzyme immobilization and micro-channel capping by the designed fabrication steps at 40oC and pressures up to 6.89 MPa.
Citation: Biomacromolecules 2008, 9, 1027–1034
Embargo: No embargo
Series/Report no.: 2008 Edward F. Hayes Graduate Research Forum. 22nd
Keywords: immobilization
lab on a chip
beta galactosidase
Description: Engineering: 3rd Place (The Ohio State University Edward F. Hayes Graduate Research Forum)
URI: http://hdl.handle.net/1811/32036
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