E2F7 and E2F8, as Potential Mediators of Rb, are Essential for Cell Survival and Embryonic Development

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dc.contributor.advisor Leone, Gustavo
dc.creator Ran, Cong
dc.date.accessioned 2007-05-25T21:47:50Z
dc.date.available 2007-05-25T21:47:50Z
dc.date.issued 2007-06
dc.identifier.uri http://hdl.handle.net/1811/25199
dc.description Graduation with Distinction en
dc.description Undergraduate Biological Science Colloqium en
dc.description.abstract E2f transcription family encompasses a wide-range of functions in regulating cell proliferation, cell differentiation and apoptosis. The two recent additions to the E2F family, E2f7 and E2f8, had unique structural features that set them apart from E2Fs. These structural features enabled these two E2Fs with the capacity for homo-dimerization and hetero-dimerization, and hence they can redundantly regulate downstream target like E2f1 and p53. Unlike the function of other E2F members, E2F7 and E2F8â s biological function in mouse development was found crucial. Simultaneous deletion of E2f7 and E2f8 induces ectopic E2F1-P53 dependent apoptosis in addition to vascular formation defect and placental deformation that eventually lead to early embryonic lethality at E11.5. My work strongly suggests that functions of E2F7 and E2F8 in the extra-embryonic cell lineages are vital for embryos to survive until term. I here show that preserving functional E2F7 and E2F8 in placental tissue allows fetuses deficient for E2f7 and E2f8 go to term, but these animals were still physiologically impaired and died soon after birth. The phenotype stemming from losses of E2f7 and E2f8 in mouse development mimicked that resulted from the loss of Rb, indicating that E2F7 and E2F8 can potentially mediate Rb in regulating cell-cycle progression and apoptosis during development and potentially during tumorgenesis. en
dc.description.sponsorship Undergraduate Student Research Scholarship en
dc.description.sponsorship NIH grants to G.L. (R01CA85619, R01CA82259, R01HD047470, P01CA097189) en
dc.format.extent 1472790 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.publisher The Ohio State University en
dc.relation.ispartofseries The Ohio State University. Department of Molecular Genetics Honors Theses; 2007 en
dc.subject E2F7 en
dc.subject E2F8 en
dc.subject Rb en
dc.title E2F7 and E2F8, as Potential Mediators of Rb, are Essential for Cell Survival and Embryonic Development en
dc.type Thesis en