OSU Navigation Bar

The Ohio State University University Libraries Knowledge Bank

Tannic Acid Effects on Raphanus Raphanistrum Root Acid Phospatase

Please use this identifier to cite or link to this item: http://hdl.handle.net/1811/23868

Show full item record

Files Size Format View
V100N5_132.pdf 389.4Kb PDF View/Open

Title: Tannic Acid Effects on Raphanus Raphanistrum Root Acid Phospatase
Creators: Yu, Karl O. A.; Fried, Colleen A.; Hall, Prudence J.
Issue Date: 2000-12
Citation: The Ohio Journal of Science. v100, n5 (December, 2000), 132-135
Abstract: The effects of tannic acid (TA) on acid phosphatase (APase) (E.C.3.1.3-2) from root extracts of fertilized or unfertilized Raphanus raphanistrum plants were examined. TA inhibited APase activity non-competitively; Vmax was reduced, but the apparent K^ was unaffected in the presence of added TA. Endogenous tannin content ranged between 0.26 and 0.56 (Imoleg fresh weight * of root tissue. Enzyme activity data was extrapolated to provide estimates of activity in the absence of any TA; uncorrected APase activity of R. raphanistrum was underestimated by an average of 25%. Addition of polyvinylpyrrolidone (PVP) did not affect measured enzyme rates in a consistent manner.
URI: http://hdl.handle.net/1811/23868
ISSN: 0030-0950
Bookmark and Share