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Lipids of Senescent Leaf Tissue Induced by Inhibition of Synthesis and Acceleration of Breakdown

Please use this identifier to cite or link to this item: http://hdl.handle.net/1811/22909

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Title: Lipids of Senescent Leaf Tissue Induced by Inhibition of Synthesis and Acceleration of Breakdown
Creators: Hancock, J. F.; Antonio, Thomas M.; Dalgarn, D. S.; Newman, D. W.
Issue Date: 1983-03
Citation: The Ohio Journal of Science. v83, n1 (March, 1983), 50-54
Abstract: Tissues were made senescent by inhibiting synthetic reactions with abscisic acid and by accelerating protein catabolism with L-serine in combination with kinetin. Squash {Cucurbita maxima) leaf discs were floated on the above-mentioned solutions, harvested, and the lipids extracted therefrom. Fatty acids of the acyl lipids were determined by gas chromatography, galactolipid concentrations were determined by sugar analyses, and phospholipid concentrations were determined by phosphorus analyses. All of the lipids were separated by thin-layer chromatography prior to analysis. Those tissues floated on abscisic acid or L-serine with kinetin contained less chlorophyll, less total fatty acids — especially linolenic acid, less glycolipid material, and less phospholipid material. Abscisic acid caused a decline in all of the cellular lipids as indicated by acyl group analysis and by analysis of the parental lipids. Possible effects of these compounds on chloroplast thylakoid stacking are discussed.
URI: http://hdl.handle.net/1811/22909
ISSN: 0030-0950
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