OSU Navigation Bar

The Ohio State University University Libraries Knowledge Bank

Gene Linkages in Phage Group 3 Staphylococcus Aureus Determined by DNA-Mediated Transformation

Please use this identifier to cite or link to this item: http://hdl.handle.net/1811/22713

Show full item record

Files Size Format View
V080N4_175.pdf 740.3Kb PDF View/Open

Title: Gene Linkages in Phage Group 3 Staphylococcus Aureus Determined by DNA-Mediated Transformation
Creators: Martin, Scott M.; Shoham, Steven C.; Alsup, Margaret; Rogolsky, Marvin
Issue Date: 1980-07
Citation: The Ohio Journal of Science. v80, n4 (July, 1980), 175-183
Abstract: A large pool of antibiotic resistant and auxotrophie mutants was isolated from the phage group 2 strains UT0002-19 and UT0017 of Staphylococcus aureus. Strain UT0002-19 has a chromosomal determinant for exfoliative toxin (ET), which causes scalded skin syndrome in man. Strain UT0017 is ET-negative. DNAmediated transformation, which employed phage 80a to induce competence, was utilized to detect gene linkages in the two strains. Three linkage groups were identified on the strain UT0017 chromosome. The first linkage group was thy-4—lys-5—trp-21— thr-4, the second was pyr-26—nov 9—his-'S, and the third consisted of ilv-9 and pen-1. Two linkage groups were detected on the strain UT0002-19 chromosome. The first was thy-1—lys-5—trp-3—thr-4:—ala-8, while the second consisted of nov-9 and his-'S. A locus for ET synthesis could not be mapped.
URI: http://hdl.handle.net/1811/22713
ISSN: 0030-0950
Bookmark and Share