Hartmannella Culbertsoni as Revealed in Scanning Electron Microscopy

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Title: Hartmannella Culbertsoni as Revealed in Scanning Electron Microscopy
Creators: Allen, Delmas J.; DiDio, Liberato John A.
Issue Date: 1976-07
Citation: The Ohio Journal of Science. v76, n4 (July, 1976), 167-171
Abstract: An axenic culture of Hartmannella culbertsoni obtained from the American Type Culture Collection was used in this study. Amoebas maintained in a trypticase soy broth medium were concentrated, fixed and dehydrated before being transferred drop-wise to round microscope cover classes for critical point drying in liquid CO2. Dried specimens were coated with carbon and palladium-gold and studied with an SEM. H. culbertsoni showed extreme polymorphism. Surface specializations or microappendages, not previously demonstrated in the light microscope, were observed. Some of the amoebas possessed smooth cellular surfaces with scattered bleb-like and bulbous ectoplasmic projections and others had irregular surface contours with threadlike processes of varying lengths. Other cells exhibited finger-like projections which formed a fringe over most of the surface. Such advanced pleomorphism, under identical environmental conditions, depends on the phase of growth, differential adhesion and locomotion of cells.
Description: Author Institution: Department of Anatomy, Medical College of Ohio
URI: http://hdl.handle.net/1811/22386
ISSN: 0030-0950
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